Error: Empty overlap set / empty target sequence set

[Krannich479]

Error message:

$ vechat <inut01>.fq -t 48 --platform ont -o <input01>.vechatCorrected.fq
Performing the 1 iteration for error correction...
Killed
perform variation graph based (haplotype-aware) error correction
[racon::Polisher::initialize] loaded target sequences 0.293457 s
[racon::Polisher::initialize] loaded sequences 0.275359 s
[racon::Polisher::initialize] error: empty overlap set!
Performing the 2 iteration for error correction...
perform linear sequence based error correction
[racon::Polisher::initialize] error: empty target sequences set!

Context:
I tried to error-correct a set of ONT reads using vechat v1.1.1 installed via bioconda and ended up with the error message above. I tried using vechat with two different sets of ONT input data, let's call them input01 and input19 here. In both cases I ended up with the error message above.

input	protocol	mean read coverage	file size
input01	amplicon seq	10059	95 MB
input19	WGS	4365	276 MB

Hence, data is present and plenty.

Question:
Why do I end up with the error message given the vechat command above? Should I adjust some parameters?

Additional:
I don't expect the files to be corrupted. Tools like canu and mosdepth work fine on the same files. Also, I already retried using vechat with different thread numbers (-t 1, 16, 48).

Related issues:

• Possibly related to errors with ONT #15 .
• Possibly related to Error- sh: fpa: command not found #3 . My worker instance got 16 GB main memory assigned. I presume that should be enough given the input file sizes?

[Axze-fire]

I wonder if vechat is still actually maintained the last released is more than 2 years old.