hi, I imputed my dataset with alra in seurat. after the imputation I integrated the alra assay and calculated pca, umap and clusters. the problem is that when I look for the top markers per cluster, using alra as an assay, I don't find anything specific for that cluster. should I use the rna assay? or what is the problem?
hi, I imputed my dataset with alra in seurat. after the imputation I integrated the alra assay and calculated pca, umap and clusters. the problem is that when I look for the top markers per cluster, using alra as an assay, I don't find anything specific for that cluster. should I use the rna assay? or what is the problem?