What problem would this solve?
Users on polarization-capable systems (e.g. Leica STELLARIS with a rotatable polarizer) want time-resolved anisotropy. The only non-commercial tool that does it (FLIMfit) supports it only from .sdt, not PTU, so there is currently no PTU-based option.
Proposed solution
Support sequential polarized acquisition: two PTUs per FOV (polarizer at 0° and 90°), background-subtract each decay, compute the anisotropy decay r(t), and fit it. Single-detector sequential acquisition, so no simultaneous dual-channel hardware is required.
Notes
- No G-factor correction needed when the same detector is used for both acquisitions.
- Theoretical r0 max = 0.4 for one-photon excitation.
- Reference: anisotropy acquisition/analysis chapter (Lakowicz) provided by the requester.
Area
FLIM fitting
Raised during external facility testing, June 2026.
What problem would this solve?
Users on polarization-capable systems (e.g. Leica STELLARIS with a rotatable polarizer) want time-resolved anisotropy. The only non-commercial tool that does it (FLIMfit) supports it only from
.sdt, not PTU, so there is currently no PTU-based option.Proposed solution
Support sequential polarized acquisition: two PTUs per FOV (polarizer at 0° and 90°), background-subtract each decay, compute the anisotropy decay r(t), and fit it. Single-detector sequential acquisition, so no simultaneous dual-channel hardware is required.
Notes
Area
FLIM fitting
Raised during external facility testing, June 2026.