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Mitchell R. Vollger
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src/quick-start.md

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The primary tool for handling Fiber-seq data is `fibertools`, and this page provides a high level order of operations for turning you raw Fiber-seq data into useful chromatin information.
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The primary tool for handling Fiber-seq data is `fibertools`, and this page provides a high level order of operations for turning you raw Fiber-seq data into useful chromatin information. The steps differ slightly depending on if you are starting with PacBio or Oxford Nanopore Technologies (ONT); however, the steps can be summarized as:
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- Create or filter m6A calls
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- Infer nucleosomes and modification sensitive patches (MSPs)
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- Align and phase the data
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- Apply Fiber-seq inferred regulatory element (FIRE) calling to identify peaks and create UCSC browser tracks
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# Fiber-seq starting with PacBio
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