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_quarto.yml
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project:
type: website
output-dir: docs
resources:
- img/
- css/
- slides/
- schedule/
website:
title: "Introduction to single-cell RNA-seq"
google-analytics: G-68JBPLLNLG
page-footer: >
This lesson has been developed by members of the teaching team at the [Harvard Chan Bioinformatics Core (HBC)](http://bioinformatics.sph.harvard.edu/). <br>
These are open access materials distributed under the terms of the [Creative Commons Attribution license](https://creativecommons.org/licenses/by/4.0/) (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. <br>
A portion of these materials and hands-on activities were adapted from the [Satija Lab's](https://satijalab.org/) [Seurat - Guided Clustering Tutorial](https://satijalab.org/seurat/pbmc3k_tutorial.html)
navbar:
left:
- text: "Schedule"
href: schedule/schedule.qmd
right:
- text: "HBC"
href: https://bioinformatics.sph.harvard.edu/
- text: "GitHub"
href: https://github.com/hbctraining/Intro-to-scRNAseq
- text: "Contact us"
href: mailto:hbctraining@hsph.harvard.edu
sidebar:
title: "Lessons" # Optional title above the sidebar
style: "docked" # Sidebar is docked to the left
collapse-level: 1 # At what header level to start collapsing
# contents: auto # Creates automatic TOC from page headers (no sections)
contents:
- section: "Pre-reading:"
contents:
- lessons/01_intro_to_scRNA-seq.qmd
- lessons/02_generation_of_count_matrix.qmd
- section: "Day 1:"
contents:
- lessons/03_quality_control-setup.qmd
- section: "Day 1 Self-learning:"
contents:
- lessons/04_cellranger_QC.qmd
- lessons/06_theory_of_PCA.qmd
- section: "Day 2:"
contents:
- lessons/05_quality_control.qmd
- lessons/07_SCT_normalization.qmd
- lessons/08_integration_cca_theory.qmd
- section: "Day 2 Self-learning"
contents:
- lessons/09_integration_code_harmony.qmd
- section: "Day 3:"
contents:
- lessons/10_clustering_cells_SCT.qmd
- lessons/11_clustering_quality_control.qmd
- section: "Day 3 Self-learning"
contents:
- lessons/12_seurat_cheatsheet.qmd
- section: "Day 4:"
contents:
- lessons/13_marker_identification.qmd
- lessons/14_scRNAseq_workflow.qmd
format:
html:
theme: simplex
css: css/styles.css
fig-align: "center"
toc: true
number-sections: false
code-block-bg: true
code-copy: true # enables copy button (default in most themes)
code-tools: true # enables the copy button!
code-line-wrap: wrap
google-analytics: G-68JBPLLNLG
prompt: true
self-contained: true
# Global Execution Defaults
execute:
echo: true # show code
eval: true # run code
warning: false # show warnings
error: false # show errors
message: false # show messages
prompt: true