[Calculator]: Add qPCR primer dilution calculator

[lichangda94-sys]

Calculator or workflow name

qPCR primer dilution calculator

Category

PCR / qPCR

Laboratory workflow need

Users often need to prepare primer working solutions from concentrated primer stocks before PCR or qPCR experiments. A calculator should help determine how much primer stock and nuclease-free water are needed to prepare a target working concentration and final volume.

Required inputs

• Primer stock concentration
• Target working concentration
• Final preparation volume
• Concentration unit
• Volume unit

Expected outputs

• Primer stock volume
• Nuclease-free water volume
• Final volume
• Warning messages for invalid or very small volumes

Formula or calculation rules

C1 × V1 = C2 × V2

Stock volume = target concentration × final volume / stock concentration

Diluent volume = final volume - stock volume

Units

Concentration: M, mM, uM, nM
Volume: mL, uL

Validation rules and warnings

• Stock concentration must be greater than target concentration
• Concentration values must be positive
• Final volume must be positive
• Warn if calculated stock volume is below 0.5 uL
• Diluent volume cannot be negative

Example calculation

Prepare 100 uL of 10 uM primer working solution from 100 uM stock.

Expected result:
10 uL stock primer
90 uL nuclease-free water

References or notes

This calculator should be implemented as transparent calculation logic with unit tests. UI integration can be added later.

Scope check

• This request is for research or laboratory workflow assistance, not clinical diagnosis or treatment decisions.
• I have not included patient data, private laboratory records, API keys, credentials, unpublished proprietary protocols, or sensitive information.