Calculator or workflow name
ELISA standard curve calculator
Category
ELISA / absorbance
Laboratory workflow need
Users often need to calculate sample concentrations from ELISA standard curve absorbance values. A calculator should help users enter standard concentrations, standard absorbance values, sample absorbance values, and dilution factors, then estimate sample concentrations with clear review warnings.
Required inputs
- Standard concentrations
- Standard absorbance values
- Sample IDs
- Sample absorbance values
- Dilution factor
- Curve fitting method
- Concentration unit
Expected outputs
- Calculated sample concentration
- Dilution-corrected concentration
- Standard curve summary
- Fit quality information if available
- Warning messages for out-of-range samples
Formula or calculation rules
Initial implementation can support a simple linear standard curve:
y = a × x + b
Where:
- x = concentration
- y = absorbance
Sample concentration:
x = (sample absorbance - b) / a
Dilution-corrected concentration:
corrected concentration = calculated concentration × dilution factor
Future versions may support 4PL fitting.
Units
Concentration: pg/mL, ng/mL, ug/mL, IU/mL, custom
Absorbance: OD value
Dilution factor: numeric multiplier
Validation rules and warnings
- Standard concentrations must be positive or zero
- Absorbance values must be numeric
- At least two standard points are required for linear fitting
- Warn if a sample absorbance is outside the standard curve range
- Warn if the curve slope is zero or invalid
- Dilution factor must be positive
- Missing sample IDs should be handled safely
Example calculation
Standards:
0, 10, 20, 40, 80 pg/mL
Absorbance:
0.05, 0.12, 0.20, 0.38, 0.72
Sample:
Sample A absorbance = 0.30
Dilution factor = 2
Expected output:
Calculated concentration from the fitted standard curve
Dilution-corrected concentration = calculated concentration × 2
References or notes
Initial implementation should start with transparent linear regression and unit tests. More advanced ELISA curve fitting such as 4PL can be discussed in a separate issue.
Scope check
Calculator or workflow name
ELISA standard curve calculator
Category
ELISA / absorbance
Laboratory workflow need
Users often need to calculate sample concentrations from ELISA standard curve absorbance values. A calculator should help users enter standard concentrations, standard absorbance values, sample absorbance values, and dilution factors, then estimate sample concentrations with clear review warnings.
Required inputs
Expected outputs
Formula or calculation rules
Initial implementation can support a simple linear standard curve:
y = a × x + b
Where:
Sample concentration:
x = (sample absorbance - b) / a
Dilution-corrected concentration:
corrected concentration = calculated concentration × dilution factor
Future versions may support 4PL fitting.
Units
Concentration: pg/mL, ng/mL, ug/mL, IU/mL, custom
Absorbance: OD value
Dilution factor: numeric multiplier
Validation rules and warnings
Example calculation
Standards:
0, 10, 20, 40, 80 pg/mL
Absorbance:
0.05, 0.12, 0.20, 0.38, 0.72
Sample:
Sample A absorbance = 0.30
Dilution factor = 2
Expected output:
Calculated concentration from the fitted standard curve
Dilution-corrected concentration = calculated concentration × 2
References or notes
Initial implementation should start with transparent linear regression and unit tests. More advanced ELISA curve fitting such as 4PL can be discussed in a separate issue.
Scope check