OADFA_MCsimulation

Monte Carlo Simulation of Sequential two-photon delayed fluorescence anisotropy

Sequential Two-Photon Delayed Fluorescence Anisotropy Simulation

Overview

This repository provides a Python-based simulation tool for calculating sequential two-photon delayed fluorescence anisotropy. The tool allows users to specify various physical properties of fluorophores and laser parameters to simulate the resulting fluorescence anisotropy decay using Monte Carlo simulation.

Features

Fluorophore Properties: Users can define the following properties:

• Fluorescence lifetime
• Quantum yield
• Extinction coefficient
• Molecular size
• And more...

Laser Properties: Users can specify the following laser parameters:

• Wavelength
• Pulse width
• Intensity
• And more...

Monte Carlo Simulation:

• The rotational Brownian motion and optically activated delayed fluorescence (OADF) of the fluorophore are calculated using Monte Carlo simulation based on the provided parameters.
• The fluorescence anisotropy decay is calculated by combining the result of rotational Brownian motion OADF.
• Fluorescence anisotropy is a bulk analysis technique, therefore users can specify the number of molecules participating in the excitation-emission cycle. Increasing the number of molecules enhances result accuracy by reducing noise, but it also extends simulation time.
• For simulating regular fluorescence anisotropy using a single excitation laser, set the intensity of the secondary laser to zero.
• A polarization analyzer can be incorporated into the system.
• The resulting anisotropy decay is fitted with an exponential decay function to calculate the rotational correlation time.
• Simulation results can be exported as CSV files.
• Imported CSV files can undergo further analysis.